Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Modified Eagles500ML
A measured mixture of chemically defined ingredients makes up a basal medium which is a type of complex media. DMEM is a medium supplemented with serum and sometimes with tryptose phosphate broth (TPB). DMEM/f-12 is a completely defined synthetic medium which is protein-free.
Boston Bioproducts Inc HEPES Buffer (1 M, pH 7.6) - 500ml
HEPES Buffer (1 M pH 7 6) - 500ml (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc PIPES Buffer (1 M, pH 7.5) - 500ml
PIPES Buffer (1 M pH 7 5) - 500ml (For Research Use and Further Manufacturing Only)
Teknova 1X Dulbeccos Phosphate Buffered Saline with 0.5% BSA. 1000mL, Sterile. 2 pack.
Phosphate-buffered saline (PBS) is a salt solution that is commonly used in physiological and biochemical experiments. It can be used to maintain pH neutrality or to buffer against changes in pH in order to provide optimal conditions for cellular growth and function. The buffering range is between pH 6.8 and 7.4.
Teknova 0.45% Saline Solution, Sterile. 500mL 4 pack.
Sodium chloride is an essential ingredient in the lab. Not only does it help regulate the pH of solutions, but it also prevents growth of bacteria and other contaminants. In addition, salt is a powerful desiccant, meaning that it can help to dry out wet samples. As a result, salt is often used in a wide variety of laboratory procedures.
Teknova 1M MOPS, pH 7.0. 1000mL, Sterile. Endotoxin Tested. 2 pack.
MOPS (3-(N-morpholino)propanesulfonic acid) is a Good’s buffer and has a pKa of 7.01, making it an ideal buffer for many biological systems at near-neutral pH. MOPS can be used in cell culture media and biopharma buffer formulations as well as in the purification of antibodies, peptides, proteins and blood components. It is nontoxic to cell culture lines.
Teknova CTAB Extraction Solution. 250mL. 4 Pack.
2.0% CTAB, 100mM Tris-HCl, pH 8.0,20mM EDTA, pH 8.0, 1.4M Sodium Chloride
United States Biological Corporation TRIS HCL BUFFER 1M PH7.5,500ML
Tris is one of the most widely used buffers in molecular biology and cell culture due to its low toxicity, stability and buffering capacity. Purity: ≥99.9% Appearance: White, crystalline powder pH (1M): 10.5-11.5 pKa: 8.0-8.4 Absorbance (260nm): ≤0.06 Absorbance (280nm): ≤0.05 Absorbance (290nm): ≤0.2 Solubility (1M): Colorless, clear, complete Loss on Drying: ≤0.2% Melting Point: 168-172°C Heavy Metals (Pb): ≤0.0001% Arsenic: ≤0.0001% Calcium: ≤0.0001% Copper: ≤0.0001% Iron: ≤0.0005% Magnesium: ≤0.0001% Insoluble Matter: ≤0.005% Residue on Ignition: ≤0.1% RNase: DNase: Protease: Meets specifications.
Chrono Log Corporation Saline (0.9%), 15mL
This item has a minimum qty of 4 per supplier requirements.
Sterile physiological saline for irrigation (0.85% or 0.9% w/v) for CHRONOPAR Reagent preparation and for dilution of the Whole Blood specimen.
Sigma Aldrich Fine Chemicals Biosciences MOPS hemisodium salt >=99% (titration) | 117961-20-3 | 250G
MOPS hemisodium salt >=99% (titration) | Purity: >=99% (titration) | Mol Wt: 220.25 | 117961-20-3 | 250G
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Phosphate Buffer
Dulbecco Phosphate Buffered Saline (DPBS) is a balanced salt SOLUTIONon.